Molecular Counting with Calibrated Labeling and Quantitative Fluorescence Microscopy
نویسندگان
چکیده
منابع مشابه
Molecular Counting with Localization Microscopy: A Redundant Labeling Approach
Super-resolved localization microscopy (SLM) has the potential to serve as an accurate, single-cell technique for counting the abundance of intracellular molecules. However, the stochastic blinking of single fluorophores can introduce large uncertainties into the final count. Here we provide a theoretical foundation for applying SLM to the problem of molecular counting in such a way as to mitig...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2020
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2019.11.1754